EBTT 2017

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought; each participant submitting an abstract was also required to give a short oral presentation of the work. The course was accredited by the Medical Chamber of Slovenia, so participants received 34 CME credits.

Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana.

Organising committee: Chair — Peter Kramar; Members — Matej Kranjc, Lea Vukanović, Tadeja Forjanič, Duša Hodžić.

Course content

Preliminary programme & topics

Electrical properties of cells & tissues

Resting & induced transmembrane voltage; dielectric properties; temperature & conductivity changes.

Physical chemistry of electroporation

Electrochemical membrane thermodynamics; molecular dynamics simulations.

Electroporation in vitro & in vivo

Protocols for cells in vitro and in vivo.

Devices & electrodes

Devices & electrodes; tissue models of electroporation in vivo and their validation.

Electrochemotherapy of tumours

Mechanisms, preclinical & clinical use, current developments & perspectives.

Gene transfection

Gene transfection in vitro/in vivo; clinical use in human medicine.

Transdermal drug delivery

Electroporation for gene delivery in the skin; DNA vaccines.

Highlights of 2017

Non-thermal plasmas (Pouvesle); reversible EP for foods (Gomez); IRE tumour ablation (Moche); MSC electromanipulation (Andre).

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič

Co-DirectorLluis M. Mir

Faculty members & lecturers

Damijan MiklavčičUniversity of Ljubljana, Slovenia
Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
Marie-Pierre RolsCNRS & IPBS, Toulouse, France
Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
Mounir TarekUniversity of Lorraine, Nancy, France
Justin TeissiéCNRS & IPBS, Toulouse, France
P. Thomas VernierOld Dominion University, Norfolk, Virginia, USA
Julie GehlHerlev Hospital, Herlev, Denmark

Invited lecturers

Franck AndreCNRS & Institut Gustave Roussy, Villejuif, France
Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
Federico GomezFood Engineering Dept., Lund University, Sweden
Tadej KotnikUniversity of Ljubljana, Slovenia
Michael MocheUniversity of Leipzig, Germany
Véronique PréatCatholic University of Louvain, Brussels, Belgium
Jean Michel PouvesleUniversity of Orleans, France

Founding faculty members

Damijan MiklavčičUniversity of Ljubljana, Slovenia
Lluis M. MirCNRS & Institut Gustave Roussy, France
Eberhard NeumannUniversity of Bielefeld, Germany
Véronique PréatCatholic University of Louvain, Belgium
Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
Justin TeissiéCNRS & IPBS, Toulouse, France

Hands-on

Practical work & exercises

Wet-lab, computer-modelling, hardware and e-learning sessions. Exercise sheets (PDF) as distributed to participants, plus the laboratory-safety sheets.

S1	Laboratory safety	PDF ↓
S2	Electroporation hardware safety	PDF ↓
L1	The influence of Mg²⁺ ions on gene electrotransfer efficiency	PDF ↓
L2	Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM	PDF ↓
L3	Electropermeabilization detection with propidium iodide in plated cells	PDF ↓
L4	Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS	PDF ↓
L5	Electrotransformation of Escherichia coli with plasmid DNA	PDF ↓
L6	Electroporation of planar lipid bilayers	PDF ↓
L7	E. coli inactivation by pulsed electric fields in a continuous flow system	PDF ↓
L8	Analysis of electric field orientations on gene electrotransfer visualization at the membrane level	PDF ↓
L10	Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments	PDF ↓
L11	Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomography	PDF ↓
C1	Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage & electrode position	PDF ↓
C2	Numerical modelling of thermal effects during irreversible electroporation treatments	PDF ↓
C3	Molecular dynamics simulations of membrane electroporation	PDF ↓
E1	Electroporation of cells and tissues — interactive e-learning	PDF ↓
H1	Measurement of electroporation pulses with oscilloscope, voltage and current probes	PDF ↓
H2	Development of pulsed power generators for electroporation	PDF ↓

Abstracts & publication

Abstract submission & proceedings

PDFInstructions for Authors PDFAbstract Submission Form DOCAbstract Template PDFJMB Author Guidelines

Participants were encouraged to submit a manuscript for a special issue of the Journal of Membrane Biology devoted to electroporation-based technologies and treatments, guest-edited by P. T. Vernier, L. M. Mir and D. Miklavčič.

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros — Mrs. Nina Bernard
nina@albatros-bled.com
tel +386 4 5780 350 · fax +386 4 5780 355