November 12-18, 2017

Ljubljana, Slovenia

Electroporation-Based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

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Practical work:

You will be assigned to practical work sessions only after you have paid the registration fee and have passed the laboratory safety test. Your chosen practical work preferences will be taken into account, until the places for practical work sessions are filled. Sessions with a limited number of participants will be filled on a first-come-first-served basis. You can select up to three practical work sessions from wet lab and modelling and both from computer simulation and e-learning. Please be sure to mark all practical work sessions by priority, as both wet and modelling sessions have limited number of participants, therefore it's possible that your second or third choice will be considered instead of your first one.

Laboratory safety test:

S1 - Laboratory safety

S2 - Electroporation hardware safety

Please Sign-in/Login to the education system (in the left navigation menu). Use equal e-mail address that you used it on registration form. In the education system, you will find Laboratory safety test course. On the left side of the menu click test. Solve the questionary and submit it.

List of exercises:

L1 - The influence of Mg2+ ions on gene electotransfer efficiency

L2 - Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM

L3 - Electropermeabilization detection with propidium iodide in plated cells

L4 - Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS

Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS on JOVE

L5 - Electrotransformation of Escherichia coli with plasmid DNA

L6 - Electroporation of planar lipid bilayers

L7 - E. coli inactivation by pulsed electric fields in a continuous flow system

L8 - Analysis of electric field orentations on gene electrotransfer visualization at the membrane level

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro on JOVE

L10 - Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments

L11 - Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomography

C1 - Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position

C2 - Numerical Modeling of Thermal Effects during Irreversible Electroporation Treatments

C3 - Molecular dynamics simulations of membrane electroporation

E1 - Electroporation of cells and tissues - interactive e-learning

H1 - Measurement of electroporation pulses with oscilloscope, and voltage and current probes

H2 - Development of pulsed power generators for electroporation